Assessment of Quantitation Linearity for Bovine Serum Albumin Using MMS

Microfluidic Modulation Spectroscopy (MMS) is a revolutionary new technology capable of highly sensitive and reproducible measurements of protein secondary structure using IR absorbance in the Amide I band (1700-1600 cm-1).

Application Note Introduction

Microfluidic Modulation Spectroscopy (MMS) is a revolutionary new technology capable of highly sensitive and reproducible measurements of protein secondary structure using IR absorbance in the Amide I band (1700-1600 cm-1). MMS is performed on the AQS3pro system from RedShift BioAnalytics, and it generates measurements over a wide range of complex backgrounds. The AQS3pro operates using a quantum cascade laser in combination with a microfluidic flow cell resulting in a wide dynamic range of 0.1 to >200 mg/mL for structural characterization, and further extended to 0.01 to >200 mg/mL for quantitation measurements.

Techniques Showcased in This Application Note Include:

  • In this App, BSA was prepared as a series and examined by MMS to measure the concentration linearity of the series.
  • Measures protein secondary structure by combining infrared spectroscopy
    with microfluidics to enhance sensitivity and accuracy
  • Achieves higher sensitivity across a wider concentration range compared
    to current FTIR techniques