Detecting Protein Conformational Change Due to Ligand Binding and Stabilization Using MMS

Ligand binding can affect the function of proteins and often causes conformational change in the protein target.

Application Note Introduction

Ligand binding can affect the function of proteins and often causes conformational change in the protein target. Since form fits function, determining the secondary structure of proteins with and without ligands is essential for a more complete understanding of how the ligand alters the protein’s function. RedShiftBio has developed a new technology called Microfluidic Modulation Spectroscopy (MMS) that measures protein secondary structure by combining infrared spectroscopy with microfluidics to enhance sensitivity and accuracy.

Techniques Showcased in This Application Note Include:

  • In this App, a protein was tested with two different ligands: one that completely stabilizes the protein against heat stress, and the other that moderately stabilizes it against heat stress.
  • Determining of the the secondary structure of proteins with and without ligands, offering  a more complete understanding of how the ligand alters the protein’s function
  • Modulating between sample and reference buffer with the flow cell utilized by  The AQS3pro 
  • Enabling realtime, highly accurate and compatable buffer subtraction with MMS,  for use with any ligand.