Monoclonal Antibody Analysis by Microfluidic Modulation Spectroscopy in a Complex Formulation Buffer (1 to 150 mg/mL)

Microfluidic Modulation Spectroscopy (MMS) enables quantitative analysis of monoclonal antibodies over a wide concentration range with high reproducibility and accuracy.

Application Note Introduction

Microfluidic Modulation Spectroscopy (MMS) is a powerful new infrared spectroscopy tool for protein structural analysis developed by RedShift BioAnalytics. This technology provides significant increases in sensitivity, dynamic range, and accuracy for determination of protein secondary structure relative to conventional mid-IR and far-UV CD techniques.

Benefits of the AQS3pro Showcased in This Application Note Include:

  • In this App, a native monoclonal antibody was measured by MMS in a complex buffer formulation at concentrations ranging from 1 to 150 mg/mL
  • Allows the use of simpler detectors without the need for liquid nitrogen cooling
  • Automatically introduces the sample protein solution and a matching reference buffer stream into a microfluidic flow cell
  • Modulates two fluids rapidly across the  Quantum Cascade Laser beam path,  producing nearly drift-free background compensated measurements