Secondary Structure Determination using Microfluidic Modulation Spectroscopy in the Presence of DMSO

Characterizing biologic drug products is crucial in drug development and the presence of organic solvents and optically active excipients typically hinders traditional characterization techniques.

Application Note Introduction

Characterizing biologic drug products is crucial in drug development and the presence of organic solvents and optically active excipients typically hinders traditional characterization techniques. RedshiftBio has developed the AQS3pro, a novel characterization system powered by Microfluidic Modulation Spectroscopy (MMS) as a solution to overcoming these obstacles. MMS combines Infrared (IR) spectroscopy with automatic sample handling and a micro-flow cell for accurate measurements with no buffer interference. The system uses rapid modulation between a sample and its reference buffer to generate a differential spectrum for the most accurate buffer subtraction. 

Techniques Showcased in This Application Note Include:

  • In this application note, lysozyme was dissolved in a buffer containing 0, 5, and 10% DMSO
  • Generates an output which is 100 times brighter than traditional FTIR light sources  by utilizing the ASQS3pro's Quantum Casade Laser as the IR light source
  • Demonstrates the ability of MMS to successfully structurally characterize the protein 
  • Results in a much greater signal-to-noise ratio and high sensitivity for accurate measurement of proteins ranging from 0.1 to over 200 mg/mL