Thermal Denaturation Analysis of Bovine Serum Albumin over Wide Concentration Range by Microfluidic Modulation Spectroscopy

Microfluidic Modulation Spectroscopy (MMS) enables quantitative analysis of monoclonal antibodies over a wide concentration range with high reproducibility and accuracy.

Application Note Introduction

Microfluidic Modulation Spectroscopy (MMS) is a revolutionary new technology capable of highly sensitive and reproducible measurements of protein secondary structure using IR absorbance in the Amide I band (1700-1600 cm-1). MMS is performed on the AQS3pro system from RedShift BioAnalytics, and it generates measurements over a wide range of complex backgrounds, including buffers that contain absorbing materials and scattering components such as reducing agents, adjuvants, surfactants, and other excipients.

Techniques Showcased in This Application Note Include:

  • In this App, MMS was used to study the heat-induced thermal denaturation of BSA samples
  • Allows the use of simpler detectors without the need for liquid nitrogen cooling
  • Differentiates small changes in secondary structure across a wide concentration range without the interference of excipients or loss of linearity at the lowest and highest concentrations
  • Delivers information about aggregation, stability, HOS, and similarity in addition to quantitation results