# Using Microfluidic Modulation Spectroscopy to Determine the Structural Effect of pH on the Peptide Hormone Insulin

Secondary structure characterization is an essential part of drug development, however, the tools for measuring secondary structure of small proteins and peptides are limited.

## Application Note Introduction

Secondary structure characterization is an essential part of drug development, however, the tools for measuring secondary structure of small proteins and peptides are limited. Microfluidic Modulation Spectroscopy (MMS), a new characterization platform that powers the AQS3pro system developed by RedshiftBio, measures protein secondary structure by combining infrared spectroscopy with microfluidics to enhance sensitivity and accuracy.

## Techniques Showcased in This Application Note Include:

•  Determines the structural effects of low pH on insulin using MMS
• Measures protein secondary structure by combining infrared spectroscopy
with microfluidics to enhance sensitivity and accuracy
• Achieves higher sensitivity across a wider concentration range compared
to current FTIR techniques