Application Notes & White Papers

Biosimilar App note

Biosimilar Structural Comparison of Commercially Sourced Reference Standards by MMS Rapidly Detects Subtle but Critical Differences to Correctly Predict Activity for Use in an ELISA Product

July 29, 2021

It was determined that many of the Bovine Serum Albumin (BSA) reference candidates did not exhibit acceptable activity after inclusion into the final product though all purchased samples shared the same name and identification. The inconsistency in activity causes delays, increases cost of manufacture, and adds an element of variability. Current in-house biophysical techniques fail … “Biosimilar Structural Comparison of Commercially Sourced Reference Standards by MMS Rapidly Detects Subtle but Critical Differences to Correctly Predict Activity for Use in an ELISA Product”

READ MORE

Biosimilar Comparison and Accelerated Stability Predictions Based on <2% Secondary Structure Differences Using Microfluidic Modulation Spectroscopy (MMS)

June 24, 2021

Biosimilar Comparison and Accelerated Stability Predictions Based on <2% Secondary Structure Differences Using Microfluidic Modulation Spectroscopy (MMS) Biosimilar structure analysis and stability predictions require instrumentation sensitive enough to detect critical small changes in secondary structure. The ability to see these structural differences between engineered biosimilars and their innovator molecules is vital to ensure successful drug approval. Biosimilar structure analysis and stability predictions … “Biosimilar Comparison and Accelerated Stability Predictions Based on <2% Secondary Structure Differences Using Microfluidic Modulation Spectroscopy (MMS)"

READ MORE
Detection of Pressure Induced Protein Aggregation Using MMS App note

Detection of Pressure-Induced Protein Aggregation Using Microfluidic Modulation Spectroscopy (MMS)

April 8, 2021

Detection of Pressure-Induced Protein Aggregation Using MicrofluidicModulation Spectroscopy (MMS) The ability to detect protein aggregation is important at all stages of drug development. Early detection of aggregation is most desirable to inform development decisions since aggregation can negatively affect the functionality of a protein. Pressure-Induced Protein Aggregation Detection Protein aggregation is a recognized signal of … “Detection of Pressure-Induced Protein Aggregation Using Microfluidic Modulation Spectroscopy (MMS)”

READ MORE
Secondary Structure Analysis: Using Microfluidic Modulation Spectroscopy to Determine the Structural Effect of pH on the Peptide Hormone Insulin

Secondary Structure Analysis: Using Microfluidic Modulation Spectroscopy to Determine the Structural Effect of pH on the Peptide Hormone Insulin

December 1, 2020

Secondary structure characterization is an essential part of drug development, however, the tools for measuring secondary structure of small proteins and peptides are limited.

READ MORE
protein conformational change

Detecting Protein Conformational Change Due to Ligand Binding and Stabilization Using MMS

December 1, 2020

Ligand binding can affect the function of proteins and often causes conformational change in the protein target.

READ MORE
FTIR protein analysis

Assessment of Quantitation Linearity for Bovine Serum Albumin Using MMS vs FTIR

December 1, 2020

Microfluidic Modulation Spectroscopy (MMS) is a revolutionary new technology capable of highly sensitive and reproducible measurements of protein secondary structure using IR absorbance in the Amide I band (1700-1600 cm-1).

READ MORE
Monoclonal antibody structure

Secondary Structure Analysis of NIST Monoclonal Antibody Reference Material 8671 by Microfluidic Modulation Spectroscopy

October 1, 2020

In 2016 the National Institute of Standards and Technology (NIST) made NISTmAb Reference Material (RM) 8671 commercially available for the evaluation of methods used to characterize the structure of monoclonal antibodies.

READ MORE
Secondary Structure Determination using Microfluidic Modulation Spectroscopy in the Presence of DMSO

Secondary Structure Determination using Microfluidic Modulation Spectroscopy in the Presence of DMSO

October 1, 2020

Characterizing biologic drug products is crucial in drug development and the presence of organic solvents and optically active excipients typically hinders traditional characterization techniques.

READ MORE
monoclonal antibodies secondary structure

Monoclonal Antibody Analysis by Microfluidic Modulation Spectroscopy in a Complex Formulation Buffer (1 to 150 mg/mL)

March 1, 2019

This note demonstrates that MMS enables quantitative analysis of monoclonal antibodies over a wide concentration range with high reproducibility and accuracy.

READ MORE
Thermal Denaturation

Thermal Denaturation Analysis of Bovine Serum Albumin over Wide Concentration Range by Microfluidic Modulation Spectroscopy

January 1, 2019

In this note, MMS was used to study the heat-induced thermal denaturation of BSA. The results show replicate measurements are very reproducible (99% similarity), and that MMS provides accurate secondary structure measurements for protein samples over a wide concentration range (1 to 100 mg/mL).

READ MORE