Structural Characterization and Temperature and Pressure Stress Comparison on Ovalbumin using MMS

Temperature-stress and melt curves are common stress conditions tested for gauging protein stability, however, melt curves can appear very different, yet produce the same Tm. Microfluidic Modulation Spectroscopy can help deconvolve the resulting structural changes that lead to protein unfolding. A comparison of temperature and pressure stress was applied to ovalbumin, showing that each stress unfolds ovalbumin differently. Understanding structural details aids in better overall drug design.