Experience the Cutting-Edge of Biomolecule Structure Characterization With Aurora TX
Minimal Sample Requirement: Produce highly reproducible data with just 50μL of sample at 0.1mg/mL concentration.
Versatile Biomolecule Characterization: Analyze the structure of many biomolecules including RNA, LNPs, proteins, peptides, antibodies, ADCs, and AAVs.
No Sample Preparation: Allows for spectral data collection under scientifically relevant conditions without the need for buffer exchange or dilution.
Automated Thermal Ramping: applies temperature stress in a repeatable and automated fashion to monitor structural change, guide decision-making and candidate ranking, and save valuable R&D time and budget.
Workspace-Efficient Design: Benchtop-friendly, all-in-one analytical unit with integrated touchscreen & intuitive graphical user interface for at-a-glance-determination of run status and results.
Full Automation: Minimizes user error, reduces training requirements, and eliminates the need for dedicated expert users.
Wide Concentration Range: Provides accurate and reproducible measurements across a broad concentration range from 0.1 mg/mL to >200 mg/mL.
Highly Buffer Tolerant: Enables structural analysis under experimentally meaningful conditions without buffer exchange.
High-Resolution Spectra: Generates high-resolution IR spectra of the Amide I region.
Comprehensive Analysis: In a single automated run, measure stability, structure, similarity, andaggregation.
User-Friendly Software: delta software provides complete automated generation of differential, background-subtracted spectra as well as repeatability, similarity and higher order structure – no spectroscopy expertise required.
Microfluidic Modulation Spectroscopy: Provides real-time background/buffer subtraction to enable wide buffer compatibility.
Discover Aurora TX for unparalleled insights into the structure and stability of RNA, Proteins and beyond.
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Aurora TX Applications
Aurora TX from RedShiftBio has a wide range of applications in biotherapeutics and biomolecular research:
RNA Analysis: Aurora TX is particularly well-suited for nucleic acid characterization, detecting structural changes in RNA due to sequence modifications, formulation changes, LNP encapsulation, and thermal stress.
Protein Characterization: This system can analyze the structure and stability of proteins, antibodies, enzymes, antibody drug conjugates, peptides, and more.
LNP (Lipid Nanoparticle) Analysis: Aurora TX can detect structural differences in RNA payloads upon LNP encapsulation and assess how different RNA-to-LNP ratios affect stability.
Viral Vector and Gene Therapy Research: The system is applicable in viral vector and gene therapy areas of biotherapeutic development.
Antibody-Drug Conjugates (ADCs) Analysis: Aurora TX can characterize the structure and stability of ADCs due to different labeling ratios (DAR), which are complex biotherapeutics.
Formulation Development: Enables the critical analysis of different formulations and how they affect biomolecule stability and structure, essential for developing effective therapeutics.
Stability Assessments: Thermal ramping establishes controlled temperature stress/stability assessments of biomolecules.
Quality Control: Aurora TX can be used throughout various stages of biopharmaceutical drug development, including quality control and lot-to-lot reproducibility measurements.
Comparative Analysis: The system enables comparison of higher-order structures, stability profiles, and similarity profiles of all manner of antibodies, proteins, peptides, RNA and more.
Adeno-Associated Virus (AAV) Characterization: Aurora TX can analyze the structure of AAVs and characterize their genomic payload, which is important in gene therapy applications.
These applications make Aurora TX an essential tool for researchers involved in biotherapeutic development and characterization, particularly in the emerging fields of RNA therapeutics and gene therapy.
What Specific Structural Changes in RNA Can Thermal Ramping in Aurora TX Detect?
Thermal ramping in Aurora TX can detect several specific structural changes in RNA:
Sequence Modification Effects: The thermal stress allows researchers to observe how different RNA sequences respond to temperature change, revealing structural alterations caused by even minor sequence modifications.
Formulation-Induced Changes: By applying thermal stress, Aurora TX can detect structural and stability differences in RNA molecules resulting from formulation modifications, which are crucial for developing effective RNA-based therapeutics.
Temperature-Dependent Conformational Shifts: The high-resolution temperature stress applied during thermal ramping enables the detection of subtle conformational changes in RNA structures as temperature increases.
LNP Encapsulation Effects: Aurora TX can reveal structural differences in RNA payloads upon Lipid Nanoparticle (LNP) encapsulation, showing how the encapsulation process affects RNA structure under thermal stress.
RNA-to-LNP Ratio Impacts: The system can detect how different RNA-to-LNP ratios affect the stability and structure of RNA molecules across a range of temperatures.
Thermal Stability Profiles: By combining thermal ramping with sensitive spectroscopic measurements, Aurora TX generates detailed stability profiles of RNA molecules, showing how their structures change and potentially unfold or denature at different temperatures.
Intermolecular Interactions: The thermal ramping feature may also reveal changes in RNA-RNA or RNA-protein interactions as temperature varies, providing insights into the stability of these complexes.
Secondary Structure Transitions: Aurora TX can detect transitions between different secondary structure elements (e.g., hairpins, loops, or duplexes) as temperature changes, offering a dynamic view of RNA structural behavior.
These capabilities make Aurora TX a powerful tool for comprehensive RNA structure analysis, enabling researchers to gain deeper insights into RNA behavior under various thermal conditions and formulations, which is essential for RNA-based drug development and other applications in molecular biology.
How Aurora TX's Thermal Ramping Improves the Characterization of RNA Structures
Aurora TX's thermal ramping capability significantly enhances the characterization of RNA structures in several ways:
High-Resolution Temperature Stress: The thermal ramping feature allows researchers to apply precise and controlled temperature changes to RNA samples, enabling the detection of subtle structural alterations with high precision and repeatability.
Automated Structural Change Detection: As the temperature changes, Aurora TX can automatically monitor and detect structural modifications in RNA molecules, providing insights into their thermal stability and conformational changes.
Sensitivity to Sequence Modifications: The thermal ramping capability allows researchers to observe how different RNA sequences respond to temperature changes, helping to identify structural differences caused by even minor sequence modifications.
Formulation Impact Assessment: By applying thermal stress, researchers can evaluate how different formulations affect RNA stability and structure, which is crucial for developing effective RNA-based therapeutics.
LNP Encapsulation Analysis: The thermal ramping feature enables the detection of structural differences in RNA payloads upon LNP (Lipid Nanoparticle) encapsulation and can reveal how different RNA-to-LNP ratios affect stability under thermal stress.
Streamlined Analysis Process: The integration of thermal ramping into the Aurora TX system automates the process of inducing and analyzing temperature-dependent structural changes, saving time and resources for researchers engaged in drug discovery and development.
Comprehensive Stability Profiling: By combining thermal ramping with Aurora TX's sensitive spectroscopic measurements, researchers can generate detailed stability profiles of RNA molecules across a range of temperatures, providing a more complete picture of their structural behavior.
Walk-Away Automation: The thermal ramping feature operates with walk-away automation, allowing researchers to set up experiments and obtain results without constant supervision, increasing efficiency in RNA characterization workflows.
By incorporating thermal ramping, Aurora TX provides a more dynamic and comprehensive approach to RNA structure analysis, offering insights into temperature-dependent behavior that are crucial for understanding RNA stability, function, and potential therapeutic applications.
Frequently Asked Questions
