Protein Titer in Minutes. Without Waiting for HPLC.
HaLCon delivers HPLC-level accuracy in 5minutes, using only 100 µL of sample.
HaLCon is RedShiftBio’s proprietary liquid chromatography system engineered to deliver rapid, accurate protein measurements without complex method development. Designed for upstream bioprocess monitoring, HaLCon replaces slow offline HPLC workflows with real-time data.
Why HaLCon?
- Get titer results in under six minutes
- Accuracy strongly correlates with offline HPLC
- Compatible with autosamplers for increased throughput
- Minimal training required – fully automated
- Compact footprint designed for GMP and development environments
Ideal For
- IgG quantitation
- Monoclonal antibody production
- Bioreactor sampling and process control
HaLCon supports continuous manufacturing goals by providing immediate, actionable data on protein expression levels—keeping workflows on track and reducing costly hold times.
Where HaLCon Fits in Your Workflow
DoE Cycles: Runmore experiments faster by eliminating HPLC bottlenecks.
Harvest Timing: Harvest at the right moment with titerresults in minutes, not hours.
Perfusion Monitoring: Spot column breakthrough in real time to prevent wasted product.
Column Loading: Load Protein A columns with precision and extend resin life.
HaLCon vs Other Protein Titer Methods
| Method | Time to Result | Accuracy | Reproducibility | Sample Volume | Ease of Use | Throughput |
|---|---|---|---|---|---|---|
| HaLCon | 5 min | HPLC-level (R²> 0.99) | High (>98%) | 100 µL | Fully automated, minimal training | High (Nest autosampler) |
| HaLCon (Protein A) | 30–120min per sample (plus queue time) | Gold standard | High | 500+ µL | Skilled operator, complex method development | Low–Medium |
| ELISA/ Immunoassay | Hours–days | High specificity | Variable (operator/lab dependent) | 50–200 µL | Moderate training, manual steps | Low |
Biosensors (BLI,Octet) | 15–30 min | Moderate (matrix-dependent) | Moderate | 50–100 µL | Moderate; training required | Medium |
Spectroscopy (Ultraviolet, absorbance, scattering) | Seconds–minutes | Low crude estimates) | Low | 50–200 µL | Easy | High (not reliable) |
