Monoclonal Antibody Analysis by Microfluidic Modulation Spectroscopy in a Complex Formulation Buffer (1 to 150 mg/mL)

APPLICATION NOTE

This Application Note Includes:

• A native monoclonal antibody was measured by MMS in a complex buffer formulation at concentrations ranging from 1 to 150 mg/mL
• The use of simpler detectors without the need for liquid nitrogen cooling
• Demonstration of how MMS automatically introduces the sample protein solution and a matching reference buffer stream into a microfluidic flow cell
• How MMS modulates two fluids rapidly across the Quantum Cascade Laser beam path,  producing nearly drift-free background compensated measurements

Monoclonal Antibodies Secondary Structure Determination

Microfluidic Modulation Spectroscopy (MMS) is a powerful infrared spectroscopy tool for protein structural analysis developed by RedShift BioAnalytics. This technology provides significant increases in sensitivity, dynamic range, and accuracy for determination of protein secondary structure relative to conventional mid-IR and far-UV CD techniques. This application note demonstrates the utility of the technique for the determination of monoclonal antibodies secondary structure at high buffer concentrations.