Physicochemical Characterization of ADCs (Antibody-Drug Conjugates):

 

Webinar Length: 1 hour
Broadcast Date: May 13, 2021
Time: On Demand
Host: LabRoots

In this webinar you will learn:

  • Analysis of the higher order structure (HOS) of therapeutic antibodies and antibody drug conjugates.
  • Study of the effects in secondary structure of conjugated proteins.
  • How the AQS3pro, with Microfluidic Modulation Spectroscopy, provides reliability, increased sensitivity and higher resolution for secondary structure analysis of biotherapeutics.

Abstract:

Antibodies are the most rapidly growing form of therapeutic. High-throughput methods of characterization are essential as companies and researchers move to develop new candidates. In this webinar, Dr. Shawn Owen discusses advanced characterization techniques to assess biophysical properties of antibody-drug conjugates (ADCs). For ADCs, the level of payload and site of conjugation are determined using LC/MS. Thermal stability and binding affinity are characterized using DSC, IR, and ITC. These methods, along with MMS, can be used for the in-depth analysis of these biologics and for routine product validation.

Speakers

Shawn Owen

Shawn C. Owens, Ph.D.
 

Assistant Professor in Pharmaceutics and Pharmaceutical Chemistry and Adjunct Professor of Biomedical Engineering, at The University of Utah
RSB LabRoot Webinar Jason Headshot

Jason Lin, Ph.D.
 

Senior Field Application Scientist, RedShiftBio

Biographies

Shawn C. Owens, Ph.D., Assistant Professor in Pharmaceutics and Pharmaceutical Chemistry and Adjunct Professor of Biomedical Engineering 

Shawn C. Owen, Ph.D. is an Assistant Professor in Pharmaceutics and Pharmaceutical Chemistry and Adjunct Professor of Biomedical Engineering; of Medicinal Chemistry, and of Internal Medicine at the University of Utah. He is also a member of the Huntsman Cancer Institute Experimental Therapeutics Program and the Nano-Institute of Utah. Dr. Owen’s research focuses on evaluating the pharmaceutic stability of antibody-based therapeutics, creating antibody-drug conjugates (ADCs) that induce self-amplifying therapeutic cascades, and engineering antibody-split enzyme systems for diagnostic applications. He has published more than thirty research papers on drug delivery and biotechnology, and his research has produced four patent applications. Dr. Owen is recognized with several prominent university teaching awards and is sought for outreach workshops by both academic and industry sponsors.

Jason Lin, Ph.D., Senior Field Application Scientist, RedShiftBio

Dr. Jason Lin is a Senior Field Application Scientist at RedShiftBio. At RedShiftBio, Jason utilizes Microfluid Modulation Spectroscopy to characterize changes in higher order structure in proteins, antibodies, peptides, and virus due to chemical and thermal stressing. Prior to joining RedShiftBio, Jason was a field Application Scientist for Wyatt Technology. At Wyatt Technology, Jason utilized dynamic, static, and electrostatic light scattering techniques to characterize the size, mass, and interaction of proteins, nanoparticles, polymers, polysaccharides, and AAVs. Prior to joining Wyatt Technology, Jason obtained his Ph.D. at the University of California, Santa Barbara (UCSB). At UCSB, Jason utilized fluorescence, ellipsometry, and atomic force microscopy to investigate how morphology impacts the diffusion length of excitons in organic solar cells.