Microfluidic Modulation Spectroscopy (MMS) for Monitoring Protein Structure During Re-Folding and Thermal Stress

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Track: Characterization of Biotherapeutics

Title: Microfluidic Modulation Spectroscopy (MMS) for Monitoring Protein Structure During Re-Folding and Thermal Stress

Protein stability is a critical quality attribute for a new biologic drug.  To investigate stability through folding and unfolding, we stressed multiple proteins both thermally and with pressure and measured the changes in the protein structure and melting point (Tm) using MMS.  We will also discuss a novel method for re-solubilizing and re-folding proteins using urea and pressure resulting in natively folded proteins whose structure matches that of the original unstressed protein.

Date and Time: Wednesday, January 17 at 03:05 PM - 03:20 PM