Primary, Secondary, and Quaternary Structure Characterization of IgG1, IgG2, IgG3, and IgG4 Fc regions

This app note investigates the increasing importance of structural analysis for antibodies, especially mAbs used in therapeutics. While crystallized antibody data is available, this study emphasizes the therapeutic relevance of analyzing antibodies in an aqueous environment. Using Microfluidic Modulation Spectroscopy (MMS), the study focused on the Fc regions of IgG1, IgG2, IgG3, and IgG4 in formulation conditions. The results reveal structurally similar yet distinguishable characteristics among these IgG subclasses, emphasizing the potential of MMS as a crucial tool for analyzing antibody structures in formulation conditions, contributing to drug development processes.