Behind the App Note: How secondary structure relates to enzyme activity: The structural differences between α-chymotrypsin and its inactive precursor chymotrypsinogen determined with Microfluidic Modulation Spectroscopy (MMS)

Proteins can be nearly identical in composition yet have very different activity. RedShiftBio Application Scientist Dr. Jan Schaefer walks us through a recent study that compares the structures of alpha-Chymotrypsin and its inactive precursor Chymotrypsinogen, which vary by only 4 amino acids.

The video covers how MMS was used to examine whether such structural differences exist in solution and if subtle protein structure differences can be used as reporters for activity, as well a  which types of applications can best utilize this information.